Biochemistry

Blood grouping Sera

Intended Use

The monoclonal test reagents are used to detect the antigens of the ABO blood group system by agglutination of human red blood cells. The human ABO blood group system is defined by presence or absence of the antigens A and/or B on the erythrocytes. The special feature of the ABO blood group system in that persons lacking the A and/or B antigens from the red blood cells regularly have antibodies in the serum directed against the missing antigen or antigens. The following table shows the principal antigens and antibodies of the ABO system:
Blood group Antigens present on the red blood cells Antibodies present in the serum
0 neither A nor B Anti-A and Anti-B
A A Anti-B
B B Anti-A
AB A and B none
The specific monoclonal antibodies of the test reagents agglutinate red blood cells possessing the homologous antigen or antigens. No agglutination indicates the absence of the relevant antigen. The results of the identification of the antigens using the test reagents must be confirmed by testing the serum with red blood cells of a known ABO blood group (see „Interpretation of test results“ below). Any difference in the results of the red blood cell antigen and the alloagglutinin test must be clarified.