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INTRODUCTION

Rapitest™ HIV 1 / 2 is a 3 generation, qualitative immunoassay for the detection of antibodies to HIV 1 and HIV 2 viruses in human serum / plasma / Whole Blood. Highly purified recombinant HIV 1 [gp41] and HIV 2 [gp 36] antigens are used for the simultaneous detection and differentiation of total antibodies to HIV 1 and HIV 2 . Test results are read visually without any instrumentation.

 PRINCIPLE:

Rapitest™ HIV 1 / 2 utilize the principle of two site sandwich immune chromatography assay on a nitrocellulose membrane, Which is coated with recombinant antigens specific to HIV 1 antibodies at test region “1” and HIV 2 antibody specific antigens at test region “2”. The control Region “C” is coated with Anti- Rabbit antibodies. Similar antigens are conjugated with Colloidal gold. When the test sample moves further on the membrane assembly, the gold conjugated recombinant antigens complexes with the HIV antibodies in the serum sample. This complex moves further on the membrane and will be trapped by the HIV 1 and 2 antigens coated on the test region “T1 & T2”, results in the formation of pink / purple colour line on the test region which confirms the presence of HIV antibodies in the sample, Absence of this band on the test region indicates no reaction. The unreacted conjugate moves further on the membrane, ends up in forming coloured control line. The control line serves to validate the test result. No band at region “C” indicates the test is “INVALID”.
Instructions for Use:
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