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INTRODUCTION

Rapitest™ HCV Test is a direct binding screening test for the detection of antibodies to Hepatitis C viruses, which causes chronic liver diseases. Recombinant HCV [NS3, NS4 & NS5] and structural core proteins antigens are employed to specifically identify HCV antibodies specifically. Test results are read visually without any instrumentation.

 PRINCIPLE:

Rapitest™ HCV is a membrane based test, which is coated with recombinant antigen [ NS3 , NS4, NS5 and Core] specific to HCV antibodies at test region “T”. The control Region “C” is coated with Protein A. Protein A Colloidal gold conjugate is used as tracer. When the test sample most further on the membrane assembly, the gold conjugated Protein A complexes with the HCV antibodies in the serum sample. This complex moves further on the membrane will be trapped by the HCV Combined antigens coated on the test region “ T ”, results in the formation of pink / purple colour line on the test region which confirms the presence of HCV antibodies in the sample, Absence of this band on the test region indicates no reaction or the absence of antibodies specific to HCV. The unreacted conjugate moves further on the membrane, ends up in forming coloured control line. The control line serves to validate the test result. No band at region “C” indicates the test is “INVALID”.
Instructions for Use:
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